The quantitative determination of <sup>13</sup>C palmitic acid using microsample technique of <sup>13</sup>C NMR spectroscopy

Liming Zhang

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The quantitative determination of ¹³C palmitic acid using microsample technique of ¹³C NMR spectroscopy

更新时间：2022-09-13

- The quantitative determination of ¹³C palmitic acid using microsample technique of ¹³C NMR spectroscopy
- Nuclear Techniques Vol. 10, Issue 8, (1987)
- 作者机构：
  
  中国科学院上海原子核研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：06 June 1986，
  
  Published：1987-08
- 稿件说明：
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Liming Zhang. The quantitative determination of ¹³C palmitic acid using microsample technique of ¹³C NMR spectroscopy[J]. Nuclear techniques, 1987, 10(8): 39-40,62.
DOI：

Liming Zhang. The quantitative determination of ¹³C palmitic acid using microsample technique of ¹³C NMR spectroscopy[J]. Nuclear techniques, 1987, 10(8): 39-40,62. DOI：

摘要

C核磁共振定量技术既可应用于

C标记化合物的结构定位，标记定量，又可间接应用于

C标记化合物的制备过程中。

J.N.Shoolery曾用

C核磁共振法测定植物油中脂肪酸组成

[1，2]

，但误差较大；后来关剑秋等人从样品的自旋—晶格弛豫时间（

）的缩短、核的NOE效应的消除及核磁共振参数设定等方面进行了进一步探讨

[3]

。我们采用微样品技术（

1.7mm、C/H双探头、取样量为毫克级）测定

C标记正十六酸，可使灵敏度增加5倍左右。

Abstract

The combined action of relexation reagent

chromium acetyl acetonate

with inversion gated control technique was studied and used to get a quantitative

C NMR spectra using microsample technique in the range of 3—5 mg. This method may be used as an alternative for obtaining

C labelled palmitic acid

which is a tracer for heart muscle studies. A good results have been obtained

in which

the enriched factor reached to 48 between C=O group at 175 ppm and - (CH)

at 29.7 ppm.

This means that

C isotope was enriched on C=0 group thus the same procedure may be applied to prepare

C labelled compounds.

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references

J. N. Shoolery , Prog. NMR Spectroscopy , 11 , 79 ( 1977 ).

J. N. Shoolery and W. L. Budde , Anal. Chem. , 48 , 1158 ( 1976 ).

关剑秋等，分析化学 , 12 , 6 , 484 ( 1984 )。

Sadtler Research Lab ., Sadtler standard cabon-13 NMR spectroscopy, S126, V21-24, 4604c , 1978 .

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The quantitative determination of 13C palmitic acid using microsample technique of 13C NMR spectroscopy

DOI：

摘要

Abstract

关键词

Keywords

references

The quantitative determination of ¹³C palmitic acid using microsample technique of ¹³C NMR spectroscopy