A multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure

Zongqin Xia; Tengchang Dai; Jianhua Zhang; Yaer Hu; Bingyao Yu; Xingrong Xu; Guanlu Huang; Gengrong Shen; Yaqiu Zhou; Hong Yu; Hanming Lu; Xunhai Li

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A multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure

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- A multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure
- Nuclear Techniques Vol. 10, Issue 8, (1987)
- 作者机构：
  
  1.Shanghai Second Medical University
  2.Shanghai Ruijin Hospital
  3.Shanghai Xinhau Hospital
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：24 November 1986，
  
  Published：1987-08
- 稿件说明：
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Zongqin Xia, Tengchang Dai, Jianhua Zhang, et al. A multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure[J]. Nuclear techniques, 1987, 10(8): 45-53.
DOI：

Zongqin Xia, Tengchang Dai, Jianhua Zhang, et al. A multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure[J]. Nuclear techniques, 1987, 10(8): 45-53. DOI：

摘要

In order to study the mechanism of the imbalance of amino acid metabolism during hepatic failure

a stable isotope tracer method for observing simultaneously the metabolic kinetics of several amino acids has been established.

N-L-Ala

（2，3-D

）-Leu and （2

3-D

）-Phe were chosen as nonessential

branched chain and aromatic amino acids. A single iv injection of 40 mg

N-Ala

20 mg deuterated Leu and 20 mg deuterated Phe was given to each human subject. Blood samples were taken just before and at different times （up to 60 min） after the injection. Total free amino acids were isolated from the plasma with a small dowex 50×8 column and converted to trifluoroacetyl derivatives. Their abundances were then analyzed with a GC-MS system and typical double exponential time course curves were found for all the three labelled amino acids. A two-pool model was designed and applied for compartmental analysis.

Significant changes were found in the kinetic parameters of Phe and Leu in patients with fulminant hepatitis or heptic cirrhosis. The half-lives of both Phe pools were longer and the pool sizes were larger than normal subjects

while the half-lives and pool sizes of Leu changed in the opposite direction. No marked change was found in Ala. The significance of intracellular imbalance of Phe and Leu metabolism was discussed. It is evident that the combination of GC-MS technique and multiple-tracers labelled with stable isotopes is of great potential for similar purposes.

Abstract

In order to study the mechanism of the imbalance of amino acid metabolism during hepatic failure

a stable isotope tracer method for observing simultaneously the metabolic kinetics of several amino acids has been established.

N-L-Ala

3-D

)-Leu and (2

3-D

)-Phe were chosen as nonessential

branched chain and aromatic amino acids. A single iv injection of 40 mg N-Ala

20 mg deuterated Leu and 20 mg deuterated Phe was given to each human subject. Blood samples were taken just before and at different times (up to 60 min) after the injection. Total free amino acids were isolated from the plasma with a small dowex 50×8 column and converted to trifluoroacetyl derivatives. Their abundances were then analyzed with a GC-MS system and typical double exponential time course curves were found for all the three labelled amino acids. A two-pool model was designed and applied for compartmental analysis.

while the half-lives and pool sizes of Leu changes in the opposite direction. No marked change was found in Ala. The significance of intracellular imbalance of Phe and Leu metabolism was discussed. It is evident that the combination of GCMS technique and multiple-tracers labelled with stable isotopes is of great potential for similar purposes.

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Keywords

references

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