Evaluation of radiation effect on tumor cells by the uptake rate of <sup>18</sup>F-FDG in vitro

WANG Lili QIN Songbing LOU Cheng XU Xiaoting ZHOU Juying

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Evaluation of radiation effect on tumor cells by the uptake rate of ¹⁸F-FDG in vitro

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- Evaluation of radiation effect on tumor cells by the uptake rate of ¹⁸F-FDG in vitro
- NUCLEAR TECHNIQUES Vol. 34, Issue 3, (2011)
- 作者机构：
  
  苏州大学附属第一医院放疗科
- 作者简介：
- 基金信息：
  
  苏州市社会发展基金(项目编号:ss0708)资助
- DOI：
  CLC： R734.2
- Published：01 March 2011
- 稿件说明：
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[1]王利利,秦颂兵,娄成,徐晓婷,周菊英.~(18)F-FDG摄取率评价肿瘤细胞辐射效应[J].核技术,2011,34(03):222-226.

WANG Lili QIN Songbing LOU Cheng XU Xiaoting ZHOU Juying. Evaluation of radiation effect on tumor cells by the uptake rate of ¹⁸F-FDG in vitro[J]. Nuclear techniques, 2011, 34(3): 222-226.
[1]王利利,秦颂兵,娄成,徐晓婷,周菊英.~(18)F-FDG摄取率评价肿瘤细胞辐射效应[J].核技术,2011,34(03):222-226. DOI：

WANG Lili QIN Songbing LOU Cheng XU Xiaoting ZHOU Juying. Evaluation of radiation effect on tumor cells by the uptake rate of ¹⁸F-FDG in vitro[J]. Nuclear techniques, 2011, 34(3): 222-226. DOI：

摘要

本文探讨18F-氟代脱氧葡萄糖（18F-FDG）摄取率评价不同肿瘤细胞辐射效应的可行性。将人非小细胞肺腺癌细胞株A549和人乳腺癌细胞株MCF-7分别给予不同单次剂量照射

24h后检测细胞对18F-FDG的摄取率;给予单次剂量8Gy照射

分别在24、48、72h和1、2和3w检测A549细胞对18F-FDG的摄取率;流式细胞仪检测细胞凋亡和周期变化;成克隆实验得出存活分数;MTT法检测细胞存活率

并与18F-FDG的摄取率进行秩相关分析。结果发现A549和MCF-7细胞对18F-FDG的摄取率随着单次照射剂量的增大而降低;其凋亡率与不同剂量照射后18F-FDG的摄取率的相关系数均为r=-0.96

P<0.005;G2/M%+S%与18F-FDG的摄取率的相关系数分别为r=0.96

0.93

P<0.01。细胞存活分数与18F-FDG的摄取率的相关系数分别为r=0.94

0.96

P<0.005。细胞单次照射8Gy后随着照射后时间的延长

A549和MCF-7细胞的摄取率逐渐增加达到峰值

后逐渐下降;其细胞存活率与18F-FDG的摄取率的相关系数分别为r=0.78

0.86

P<0.05。18F-FDG摄取率能较好地反映电离辐射效应

可用来评价电离辐射对肿瘤细胞的杀伤作用。

Abstract

For evaluating the potential of 18F-FDG uptake rate to monitor biological responses of irradiated tumor cells

18F-FDG uptake rates were detected for A549 and MCF-7 cells irradiated by 6 MV X-rays to

8 and 10 Gy single dose.Also

18F-FDG uptake rates of A549 and MCF-7 cells after 8 Gy single dose irradiation were detected at 1

5 and 6 d

and 1

2 and 3 w.The cell survival rate was detected by MTT.The cell cycles and apoptosis rate were detected by flow cytometry to evaluate tumor cell growth inhibition by ionizing radiation.Clonogenic assay was used to measure the survival fraction.The data were analyzed by the statistical software SAS8.0.The results showed that 18F-FDG uptake rate of A549 and MCF-7 cells decreased with increasing single doses.The 18F-FDG uptake rate after irradiation of different doses was negatively correlated with the apoptosis rate(r =– 0.96

P<0.005)

and positively correlated with G2/M%+S% and survival fraction(r =0.96;0.93

P <0.01.r =0.94;0.96

P <0.005).At different time after the single exposure of 8 Gy

the 18F-FDG uptake rate of A549 and MCF-7 cells gradually increased to reach the peak

then decreased.The cell viability was positively correlated with 18F-FDG uptake rate(r=0.78;0.86

P<0.05).It can be concluded that 18F-FDG uptake rate correlated with the changes of biology effect of tumor cells after irradiation

and it may be used to evaluate for irradiation effects of tumor cells.

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Evaluation of radiation effect on tumor cells by the uptake rate of 18F-FDG in vitro

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摘要

Abstract

关键词

Keywords

references

Evaluation of radiation effect on tumor cells by the uptake rate of ¹⁸F-FDG in vitro