In this work

we explored the method of radiolabeling scorpion toxin peptide BmK CT

and investigated the stability

the ability of cell conjugation of 131I-BmK CT

and its distribution and imaging in glioma-bearing Wistar rats.After cloning

expression

and purification

BmK CT was labeled with 131I by indirect labeling(Bolton -Hunter method).131I-BmK CT was injected into the tail vein of glioma-bearing rats

and imaged at 1

4

8

24 and 48 h.The rats were sacrificed by cervical dislocation

and tissue samples were studied.131I-BmK CT was successfully prepared with the overall yield of 37.8 ±8.9%.The radiochemical purity was more than 95%.The specific binding efficiency of 131I-BmK CT with C6 glioma cell was 39.62%.In the first 24 h after injection

the bio-distribution in rats showed rapid blood clearance.Most of the radioactivity was observed in liver

lung and kidney.After 24 h

the bio-distribution showed renal clearance

especially at the peak time of accumulation

when the tumor could be viewed clearly.The largest uptake ratio of tumor to non-tumor(background)(T/NT) at 4 h was 6.79 ±0.29.In conclusion

131I-BmK CT can be prepared and specifically conjugate with C6 glioma cell.Good bio-distribution was observed in glioma-bearing rats.Nevertheless

great deal of research work needed to be done before clinical application of the medicine.