Study on CT DNA in vitro irradiated with MeV fluorine ions

LAI Jiangnan1 XUE Jianming1 QIN Huaili1 CHEN Guohua2 WEI Xionghui2 WANG Yugang1

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Study on CT DNA in vitro irradiated with MeV fluorine ions

更新时间：2021-03-12

- Study on CT DNA in vitro irradiated with MeV fluorine ions
- NUCLEAR TECHNIQUES Issue 9, (2005)
- 作者机构：
  
  1. 北京大学重离子物理教育部重点实验室
  2. 北京大学化学与分子工程学院
  3. 北京大学重离子物理教育部重点实验室,北京,100871
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助(10375005,10435020)
- DOI：
  CLC： Q691
- Published：01 September 2005
- 稿件说明：
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[1]赖江南,薛建明,覃怀莉,陈国华,魏雄辉,王宇钢.小牛胸腺DNA的MeV重离子辐照研究[J].核技术,2005(09):678-680.

LAI Jiangnan1 XUE Jianming1 QIN Huaili1 CHEN Guohua2 WEI Xionghui2 WANG Yugang1. Study on CT DNA in vitro irradiated with MeV fluorine ions[J]. Nuclear techniques, 2005, (9): 678-680.
[1]赖江南,薛建明,覃怀莉,陈国华,魏雄辉,王宇钢.小牛胸腺DNA的MeV重离子辐照研究[J].核技术,2005(09):678-680. DOI：

LAI Jiangnan1 XUE Jianming1 QIN Huaili1 CHEN Guohua2 WEI Xionghui2 WANG Yugang1. Study on CT DNA in vitro irradiated with MeV fluorine ions[J]. Nuclear techniques, 2005, (9): 678-680. DOI：

摘要

重离子辐照DNA分子的研究都集中在SSB和DSB方面

研究辐照产生的除SSB和DSB以外的产物对研究相关的机理很重要

特别是检测组成DNA的基本单元

如碱基

核苷等的生成量和生成规律。本实验用琼脂糖凝胶电泳验证了小牛胸腺DNA辐照产物分片断主要集中在831bp附近

用MALDI-TOF检测证明在1000—30000质荷比范围内没有可检测到的生成物

HPLC分析表明有生物小分子生成

在小分子到链断裂碎片间没有或很少其它产物

说明MeV离子轰击DNA分子造成的损伤并非随机的。

Abstract

The study on products of CT DNA irradiated with heavy ion is very important to understand the biologic effect induced by heavy ions. We have analyzed the products of CT DNA irradiated with MeV F ions using agarose gel electrophoresis

MALDI-TOF and HPLC methods. The results show that the products are mostly multi-DSB in our experiment observed by agarose gel electrophoresis

and there are not observable products between 1

000 and 30

000 (m/z) using MALDI-TOF method. HPLC result indicates some small biomolecules occurring in the products. These results suggest that the break of CT DNA induced by MeV heavy ions irradiation was not a random process.

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