Mutagenic effects of wheat callus induced by <sup>60</sup>Co γ-ray irradiation

LI Minghao; HOU Jinyan; WU Lifang

doi:10.11889/j.0253-3219.2013.hjs.36.120203

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Mutagenic effects of wheat callus induced by ⁶⁰Co γ-ray irradiation

更新时间：2021-03-05

- Mutagenic effects of wheat callus induced by ⁶⁰Co γ-ray irradiation
- NUCLEAR TECHNIQUES Vol. 36, Issue 12, Article number: 120203(2013)
- 作者机构：
  
  中国科学院合肥物质科学研究院中国科学院离子束生物工程学重点实验室
- 作者简介：
- 基金信息：
  
  作物生物学国家重点实验室开放基金(2012KF09);中国科学院技术生物所联合青年基金(Y29LH24892)资助
- DOI：10.11889/j.0253-3219.2013.hjs.36.120203
  CLC： S512.1
- Published：01 December 2013
- 稿件说明：
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[1]李明浩,侯金艳,吴丽芳.~(60)Coγ射线对小麦愈伤组织的诱变效应研究[J].核技术,2013,36(12):21-26.

LI Minghao, HOU Jinyan, WU Lifang. Mutagenic effects of wheat callus induced by ⁶⁰Co γ-ray irradiation[J]. Nuclear techniques, 2013, 36(12): 120203
[1]李明浩,侯金艳,吴丽芳.~(60)Coγ射线对小麦愈伤组织的诱变效应研究[J].核技术,2013,36(12):21-26. DOI： 10.11889/j.0253-3219.2013.hjs.36.120203.

LI Minghao, HOU Jinyan, WU Lifang. Mutagenic effects of wheat callus induced by ⁶⁰Co γ-ray irradiation[J]. Nuclear techniques, 2013, 36(12): 120203 DOI： 10.11889/j.0253-3219.2013.hjs.36.120203.

摘要

创造新的种质资源材料

对于加速小麦遗传改良研究具有重要意义。利用60Coγ射线对小麦幼胚愈伤组织进行5-30 Gy剂量的辐照处理

并对其生物学效应进行研究。结果表明

辐照处理对小麦幼胚愈伤组织的分化产生很大影响

随辐照剂量增大

愈伤组织的分化率逐渐下降。对M2代分离群体进行观察

获得叶片、茎秆、株型和穗型等形态性状发生变异的突变体

以及生育期等生理性状发生改变的变异株。这些突变体材料为小麦新品种的选育及其功能基因组学研究提供了很好的基础材料。

Abstract

Background: Creation of new germplasm derived from wheat can accelerate the genetic breeding of wheat. Purpose: Samples of the callus from immature embryo were irradiated by 60Co γ-ray to study their biological effects. Methods: The calluses were irradiated at the doses of 5 Gy

10 Gy

15 Gy

20 Gy and 30 Gy(dose rate 10 Gy/min). And the physiological characters in M2 generation were observed. Results: The results showed that the rates of callus differentiation decreased with the increasing of doses. Morphological variations including leaf

stalk

plant type

panicle type and various physiological characters were observed in the M2 generation. Conclusion: The mutants were helpful for gene identification and gene functional analysis

and for breeding of new wheat variety as well.

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Mutagenic effects of wheat callus induced by 60Co γ-ray irradiation

DOI：10.11889/j.0253-3219.2013.hjs.36.120203

摘要

Abstract

关键词

Keywords

references

Mutagenic effects of wheat callus induced by ⁶⁰Co γ-ray irradiation