Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography

CHEN Huabin; HU Jianzhong; ZHOU Jingyong; ZHENG Cheng; WANG Zhanwen; CHEN Can; CAO Yong; YIN Xianzhen; LYU Hongbin

doi:10.11889/j.0253-3219.2015.hjs.38.110101

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Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography

更新时间：2021-03-03

- Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography
- NUCLEAR TECHNIQUES Vol. 38, Issue 11, Article number: 110101(2015)
- 作者机构：
  
  1. 中南大学湘雅医院运动医学科中南大学运动医学研究中心,长沙,410008
  2. 中南大学湘雅医院脊柱外科,长沙,410008
  3. 武汉体育学院医院,武汉,430079
  4. 中国科学院上海药物研究所药物释放系统研究中心,上海,201203
- 作者简介：
- 基金信息：
- DOI：10.11889/j.0253-3219.2015.hjs.38.110101
  CLC：
- Published：13 November 2015
- 稿件说明：
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CHEN Huabin, HU Jianzhong, ZHOU Jingyong, et al. Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography[J]. Nuclear techniques, 2015, 38(11): 110101
DOI：

CHEN Huabin, HU Jianzhong, ZHOU Jingyong, et al. Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography[J]. Nuclear techniques, 2015, 38(11): 110101 DOI： 10.11889/j.0253-3219.2015.hjs.38.110101.

摘要

利用同步辐射X射线相衬成像技术(Synchrotron Radiation-based X-ray Phase-contrast Computed Tomography

SR-XPCT)对兔髌骨-髌腱连接点(Patella-Patellar Tendon Junction

PPTJ)移行区的纤维软骨细胞进行三维可视化及量化表征。正常骨成熟的新西兰PPTJ经多聚甲醛固定、梯度酒精脱水后，于上海光源X射线成像与生物医学应用光束线站进行扫描；采集的原始图像经数字减影、相位恢复、切片重构、位数转换后进行三维重构分析；标本扫描后进行传统组织学验证。SR-XPCT能成功获取PPTJ及其移行区纤维软骨细胞的三维高清图像，并对移行区纤维软骨细胞进行了量化表征：平均直径为(10.139±1.265) ?m；平均体积为(291.187±87.283)μm3，体积分布在200−400 μm3之间的占75.4%；平均球形度为0.711±0.079，球形度在0.605−0.805之间的纤维软骨细胞约占84.9%。SR-XPCT作为一种先进的、直观准确的可视化骨腱连接点的三维微观形态结构研究方法，为骨腱连接点损伤修复的三维形态学研究提供了新方法、新思路。

Abstract

Background: Synchrotron radiation-based X-ray phase-contrast computed tomography (SR-XPCT) is an advanced tool for nondestructive analysis of three dimensional inner microstructure of samples

and plays an important part in the researches such as spinal cord microvasculature and lung bronchium. Purpose: This study was to investigate the three-dimensional morphology of fibrochondrocytes in rabbit patella-patellar tendon junction (PPTJ) using SR-XPCT

and develop an advanced imaging method. Methods: Three PPTJs were harvested from healthy mature female New Zealand rabbits. The specimens were scanned by SR-XPCT at BL13W1 of Shanghai Synchrotron Radiation Facility (SSRF) in China. The tomographic image was captured by Charge Coupled Device (CCD) detector with a 0.65-μm resolution. The three-dimensional visualization images of PPTJ were reconstructed by VG Studio Max. After that

the specimens were embedded by paraffin for Safranin O/Fast-green staining. Results: High-resolution three-dimensional visualization images of PPTJ and the parameters of the chondrocytes in fibrocartilage zone were successfully obtained via the SR-XPCT. With spherical and ellipsoidal morphologic features and its configuration characteristics in three dimension

the fibrochondrocytes revealed by SR-XPCT were consistent with the results of traditional histological staining. The parameters of chondrocytes showed that the mean diameter of chondrocyte was (10.139±1.265) μm

the mean volume was (291.187±87.283) μm3 and 75.4% of the chondrocytes’ volume ranged from 200 μm3 to 400 μm3

the mean sphericity degree was 0.711±0.079

and 84.9% of chondrocytes’ sphericity degree ranged from 0.605 to 0.805. Conclusion: The three-dimensional microstructure of the PPTJ was intuitively

accurately and undistortedly realized by the SR-XPCT

which may act as a novel instrument to access the three-dimensional microstructure changes during the PPTJ healing process.Cited

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