Compression elastic property of DNA origami measured by atomic force microscopy

Guokai YU; Yuhui WEI; Lin LIU; Ping ZHANG; Shuo WANG; Wenjing LIU; Xingfei ZHOU; Bin LI

doi:10.11889/j.0253-3219.2017.hjs.40.040501

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Compression elastic property of DNA origami measured by atomic force microscopy

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- Compression elastic property of DNA origami measured by atomic force microscopy
  封面论文
- NUCLEAR TECHNIQUES Vol. 40, Issue 4, Article number: 040501(2017)
- 作者机构：
  
  1.宁波大学理学院宁波 315211
  2.中国科学院上海应用物理研究所物理生物学研究室嘉定园区上海 201800
  3.中国科学院大学北京 100049
- 作者简介：
- 基金信息：
  
  National Basic Research Program of China;National Basic Research Program of China;National Natural Science Foundation of China;National Natural Science Foundation of China;National Natural Science Foundation of China;Chinese Academy of Sciences Knowledge Innovation Project
- DOI：10.11889/j.0253-3219.2017.hjs.40.040501
  CLC： TL99
- Received：17 January 2017，
  
  Revised：15 February 2017，
  
  Published：2017-04
- 稿件说明：
移动端阅览
Guokai YU, Yuhui WEI, Lin LIU, et al. Compression elastic property of DNA origami measured by atomic force microscopy[J]. Nuclear techniques, 2017, 40(4): 040501
DOI：

Guokai YU, Yuhui WEI, Lin LIU, et al. Compression elastic property of DNA origami measured by atomic force microscopy[J]. Nuclear techniques, 2017, 40(4): 040501 DOI： 10.11889/j.0253-3219.2017.hjs.40.040501.

摘要

原子力显微术（Atomic force microscopy，AFM）的力学成像模式可在高分辨成像的同时，定量测量材料的力学性质。然而，对尺度小、质地薄而软的生物分子的弹性模量的测量仍然是一个挑战。本文以脱氧核糖核酸（Deoxyribonucleic acid，DNA）折纸为检测样品，将峰值力定量纳米力学模式（Peak Force Quantitative Nanomechanical Mapping，PF-QNM）作为测量手段研究了DNA分子的力学性质，探索不同作用力对DNA折纸弹性模量的影响。结果表明，当峰值力控制在80-100 pN时，峰值力成像稳定，获得的杨氏模量维持在约10MPa。与传统力曲线阵列模式（Force volume mapping，FV）相比较，在小力区（ < 100 pN），两种方法符合性较好。这种峰值力定量纳米力学模式为DNA分子定量力学性质研究提供了一种简便而有效的研究方法。

Abstract

Background

The mechanical mapping mode of atomic force microscopy (AFM) enables to measure mechanical properties of materials while imaging with high-resolution. However

to measure the elastic Young's modulus of biomolecules is still a challenge because they are so small

soft and thin.

Purpose

This study aims to explore the compression elasticity of DNA origami and the different forces on the effect of Young's measurement on the deoxyribonucleic acid (DNA) origami sample.

Methods

The peak force imaging mode (Peak Force Quantitative Nanomechanical Mapping

PF-QNM) was used to measure the Young's modulus of DNA origami under various activing forces.

Results

It was found that by using of 80-100pN peak forces

the elastic Young's modulus measurement results were relatively stable

keeping about 10 MPa for the peak force measurement on DNA origami. Compared with the traditional force volume contract mode (Force volume mapping

FV)

the values obtained consisted well with that of FV when the force were limited below 100 pN.

Conclusion

This method provided a simple and effective way for quantitative measuring the elasticity of DNA molecules.

关键词

Keywords

references

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