Electrospinning sample delivery for serial crystallography using synchrotron radiation

Jianqiao CHEN; Qisheng WANG; Bing LI; Huan ZHOU; Ying CUI; Bo SUN; Zhijun WANG; Jianhua HE

doi:10.11889/j.0253-3219.2017.hjs.40.080102

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Electrospinning sample delivery for serial crystallography using synchrotron radiation

SYNCHROTRON RADIATION TECHNOLOGY AND APPLICATIONS | 更新时间：2021-05-19

- Electrospinning sample delivery for serial crystallography using synchrotron radiation
- NUCLEAR TECHNIQUES Vol. 40, Issue 8, Article number: 080102(2017)
- 作者机构：
  
  1.中国科学院上海应用物理研究所张江园区上海 201204
  2.中国科学院大学北京 100049
- 作者简介：
- 基金信息：
  
  Strategic Priority Research Programmes (B) of Chinese Academy of Sciences;Youth Innovation Promotion Association of Chinese Academy of Sciences;National Natural Science Foundation of China
- DOI：10.11889/j.0253-3219.2017.hjs.40.080102
  CLC： TL99
- Received：11 April 2017，
  
  Revised：28 April 2017，
  
  Published：10 August 2017
- 稿件说明：
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Jianqiao CHEN, Qisheng WANG, Bing LI, et al. Electrospinning sample delivery for serial crystallography using synchrotron radiation[J]. Nuclear techniques, 2017, 40(8): 080102
DOI：

Jianqiao CHEN, Qisheng WANG, Bing LI, et al. Electrospinning sample delivery for serial crystallography using synchrotron radiation[J]. Nuclear techniques, 2017, 40(8): 080102 DOI： 10.11889/j.0253-3219.2017.hjs.40.080102.

摘要

串行晶体学作为一种解析蛋白质晶体结构的新方法，因其拥有室温采集、辐射损伤低、时间分辨等优势而得到迅速发展。早期发展于自由电子激光的串行飞秒晶体学已成功实现了蛋白质微小晶体结构的解析。为了引入串行晶体学的技术优势，充分发挥第三代同步辐射的丰富资源和特点，基于上海光源BL17U1生物大分子线站，我们对串行晶体学上样方法进行了理论和实验研究，解决了静电纺丝上样方式在同步辐射上应用的难题，用溶菌酶微小晶体论证了其可行性；同时探讨了基于同步辐射的串行晶体学在毫秒时间尺度上的发展潜力，为光束线技术升级提供参考。

Abstract

Background

As a novel approach to solve the protein structure

serial femtosecond crystallography (SFX) has been developed rapidly owing to the advantages of the room-temperature data collection

lower radiation damage and time-resolved study. The first X-ray free-electron laser (XFEL) based SFX experiment has offered a new method to structure determination of protein microcrystals successfully.

Purpose

The aim is to introduce SFX into the widely-available third-generation synchrotron radiation (SR).

Methods

The electrospinning sample delivery system is a potential method to be applied on SR. The theoretical and experimental researches on electrospinning were studied using BL17U1 beamline in Shanghai synchrotron radiation facility (SSRF).

Results

A microjet containing microcrystals was produced from the liquid meniscus and controlled by an electrostatic field. The air ionization problem disturbing electrospinning in SR experiment was solved by providing vacuum condition and coaxial electrospinning technique. Using lysozyme microcrystals as a model sample

the feasibility of electrospinning was demonstrated.

Conclusion

It is shown that the serial crystallography on millisecond time scale is suitable for SR

which provides a reference for the future beamline upgrading.

关键词

Keywords

references

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